EXPLORATION AND COLLECTION OF PLANT GENETIC RESOURCES DURING 2021-22
M M ALI, M S NAHAR, R AFROZ, N PERVIN, S RAHMAN, M G HOSSAIN, I AHMED,
M R MOLLA, N JAHAN, M F KHATUN, Q M AHMED AND N JAHAN
Multi-crop exploration and collection program was undertaken in 31upazilas of 19 districts in Bangladesh during 2021-2022. Five hundred and seven (507) germplasm of 63 crops were collected from Bogura, Chapainawabganj, Chattogram, Chuadanga, Coxsbazar, Dhaka, Faridpur, Gazipur, Jamalpur, Jashore, Kurigram, Khustia, Lalmonirhat, Narayanganj, Narsingdi, Natore, Pabna, Rangamati and Rangpur. The germplasm were 6 cereals, 12 pulses, 13 oilseeds, 360 vegetables, 97 spices, 12 fruits, 7 other crops. These germplasms were collected from home garden, field, threshing floor, farm store, cultivated habitat, market and hilly area etc. The samples were collected as seeds, seedlings, fruits from individual plant or population. Passport data like collector’s number, local/ cultivar name, cultural practices, date of collection, donor’s name, name of village, union, upazila and district also GPS reading of the locations were recorded during germplasm collection. The samples were registered in germplasm collection register of PGRC and conserved in active collection following appropriate procedures.
CHARACTERIZATION OF HYACINTH BEAN GERMPLASM
Q M AHMED, R AFROZ, S RAHMAN, M G HOSSAIN, M R MOLLA, N JAHAN,
M F KHATUN, N JAHAN AND M M ALI
The experiment was conducted on hyacinth bean (Lablab purpureus L. Sweet) at Plant Genetic Resources Centre (PGRC) of Bangladesh Agricultural Research Institute (BARI), Gazipur, during October 2021 to May 2022 to estimate the characterization and variability in the germplasm. The germplasm was grown in augmented RCBD design including 67 germplasm with three check BARI released varieties. Green and purple colors were found in hypocotyl, epicotyl, stem pigmentation and leaf vein among the germplasm. Leaf anthocyanin was observed in 28.57% among the germplasm. Variations were observed in ramification index and stem pigmentation. Different pod shapes and pod curvatures were found. Variations of fresh seed color and dry seed color such as Green cream, purple, brown and black were discovered. The higher amount of coefficient of variations was observed in the number of pods per rachis. Phenotypic coefficient of variance (PCV) was significantly higher than the corresponding genotypic coefficient of variation (GCV) for all the character. Heritability % range from 12.88 to 96.4. Most of the characters were shown high heritability except the lowest heritability was found in leaf length. The highest genetic advance was found in number of pods per plant. High heritability coupled with high genetic advance was obtained for number of pods per plant, number of rachis, harvest duration and total seed weight. High heritability coupled with low genetic advance was observed for seed breadth, flower bud length, edible pod length, and yield per plot. Some promising germplasm were found.
CHARACTERIZATION OF ASH GOURD GERMPLASM DURING 2021-22
Q M AHMED, R AFROZ, S RAHMAN, M G HOSSAIN, M R MOLLA, N JAHAN,
M F KHATUN, N JAHAN AND M M ALI
The present experiment was conducted with 41 germplasm of ash gourd (Benincasa hispida Thumb.) at the Plant Genetic Resources Centre (PGRC) of Bangladesh Agricultural Research Institute (BARI) during the Kharif season 2021-22. Germplasm was varied for seventeen qualitative and eleven quantitative characters. Qualitative characters of ash gourd variation were revealed in early plant vigor, plant growth habit, leaf, inflorescences and fruit characters. The monoecious flower was recorded in all of the germplasm. Based on the fruit qualitative characters’ maximum variations were obtained in fruit shape as well as stem-end and blossom-end fruit shape. The early female flowering (35 days) germplasm was found in AC-256, AC-278 and AC-433 followed by TRMR-2 as well as late was found in TRMR-147 (53 days) followed by NSR-56. The highest fruit length was obtained by the germplasm AC-96/1 (24.75 cm) followed by AC-61 (23 cm). The fruit width ranged from 9.0 to 12.50 cm and an average of 10.71 cm. The highest individual fruit weight was found in germplasm AC-256 (3.20 kg) followed by AM-25 (1.45 kg). The highest flesh thickness was recorded in AC-117 (2.40 cm) followed by AM-25 and AC-454 whereas the lowest in MRI-21 (1.00 cm) followed by AC-223 (1.33 cm). Based on recorded data it was observed a wide range of variations were found in most of the traits which indicate scope for breeding planning.
CHARACTERIZATION OF ASH GOURD GERMPLASM DURING 2020-21
Q M AHMED, R AFROZ, S RAHMAN, M G HOSSAIN, M R MOLLA, N JAHAN, M F KHATUN, N JAHAN AND M M ALI
The experiment was conducted with 36 germplasm of ash gourd (Benincasa hispida Thumb.) at the Plant Genetic Resources Centre (PGRC) of Bangladesh Agricultural Research Institute (BARI) during the Kharif season 2020-21. Germplasm was varied for different qualitative and quantitative characters. Twenty-one qualitative characters of ash gourd variation was revealed in early plant vigor, plant growth habit, leaf and fruit characters as well as seediness. Monoecious flower was recorded in all of the germplasm. The earlier female flower and the longest petiole length was found in ZS-05. Maximum fruits per plant were found in AMA-316 and AMA-62. The highest fruit length, weight and yield per plant were recorded in RC-197 close to AMA-71. Germplasm were grouped into four clusters. Cluster I, II, III, and IV contained member 6, 10, 6 and14, respectively. Cluster I had the highest mean values for germination percent, peduncle length, Number of primary branches, fruit length and individual fruit weight. Cluster II had the highest mean values for number of fruits per plant, fruit breadth and yield per plant. Sex ratio was maximum in cluster III and the nearest nodal position and earlier flower appearing was observed in female flower. Principal components analysis (PCA) revealed first eight most informative components PC8 cumulative variance 84.91 % whereas PC1 22.21%. Therefore, selection of these genotypes might play a significant role for future breeding program.
CHARACTERIZATION OF BOTTLE GOURD GERMPLASM
R AFROZ, M G HOSSAIN, M F KHATUN AND M S NAHAR, M M ALI
Characterization and genetic variability for 30 characters in 98 accessions of bottle gourd (Lagenaria vulgaris) were studied. All accessions showed variation in qualitative and quantitative traits. Highly significant differences were observed among the quantitative traits. In qualitative traits, variability was found in fruit shape, pre-dominant fruit skin color, secondary fruit skin colour, plant vigor, stem pubescence, leaf colour, leaf size, depth of leaf lobes on leaf margin, leaf pubescence on dorsal and ventral part, fruit firmness, seed size, seed colour and flower colour. In quantitative traits, the highest mean value was observed in case of days to fruit maturity (128 days) followed by days to green fruit harvest (128 days) and days to first female flower (101days). The lowest mean value (5) was for number of fruit per plant. The highest standard deviation (SD) was found in days to green fruit harvest (13.49) and the lowest in weight per fruit in kilogram (0.94). The maximum coefficient of variation (CV) (45%) was obtained from number of fruit per plant followed by total number of female flower per plant (31.85%), number of primary branch per plant (29.30%) and fruit length in centimeter (26.71%).
CHARACTERIZATION OF SPONGE GOURD GERMPLASM
M G HOSSAIN, M R MOLLA, I AHMED, N JAHAN, M F KHATUN, Q M AHMED, S RAHMAN, R A CHHANDA AND M M ALI
Characterization of available germplasm helps to estimate diversity among the germplasm which is the key elements to develop new improved crop varieties. To identify the important traits of collected 56 sponge gourd (Luffa cylindrica) germplasm, a characterization experiment was carried out at PGRC research field, BARI, Gazipur during kharif 2021-22 season. Variation in Qualitative and quantitative characters were observed in all recorded traits except stem shape, presence of tendrils and flower color. A good number of variations in stem pubescence, leaf shape and leaf size were observed. Different leaflet size viz. small for 10 accession (17.86%), intermediate for 30 accessions (53.57%), and large for 16 accession (28.57%) were recorded. Variation among the germplasm for leaf shape such as; ovate, orbicular and reniform were found in 4 (7014%), 8 (14.29%) and (78.57%) accessions respectively. Dissimilarity in stem pubescence like dense pubescence appeared on 8 (14.29%) and it was thin in 18 (32.14%) accessions while 30 accessions did not produce any pubescence. Significant variations were observed in respect of days to emergence, days to 1st flowering, internode length, lateral shot, peduncle length, petiole length and stem pubescence among the tested accessions. Early flowering was observed in accessions AR-260, TRMR-231 and TT-104 (11 DAT) while it was late in TT-59 (57 DAT) that took 57 days to flower. Internode length varied from 8.6 to 14.4 cm where, 18 accessions may consider as short statured (8.6−9.8 cm) and 3 accessions as tall (13-14.4cm). NQ-76, TT-59 and NSR-149 produced the highest number of lateral shoots (6-7) and the lowest were NRI- 90 and TRMR-112 which produced only 2 shoots. Petiole and peduncle length also showed a huge variation among the tested accessions.
CHARACTERIZATION OF RIDGE GOURD GERMPLASM
M F KHATUN, N JAHAN, M J HOSSAIN, S RAHMAN AND M S NAHAR
To know about the genetic diversity of 43 germplasm of ridge gourd, an experiment was conducted at the experimental field of Plant Genetic Resources Centre (PGRC) of Bangladesh Agricultural Research Institute (BARI), Gazipur, during the Kharif season of 2021-22. Variations were found in both qualitative and quantitative characters among the germplasm. In the qualitative character variations were found in cotyledon size, cotyledon color, leaf size, leaf lobe, dorsal leaf pubescence, ventral leaf pubescence, blossom end fruit shape, stem end fryit shape, fruit shape, fruit rib, fruit color, flesh color, flesh taste, flesh moisture, flesh texture and skin hardness except stem shape, tendril, leaf spot colour, leaf margin, growth habit, flower colour, sex type, skin texture and flesh test. The maximum (Six types) variation was found in fruit shape which was the elliptical shape (44.19%), elongate elliptical (18.60%), pyriform (13.95%), elongate slim (9.30%), elongate tapered (6.98%) and oblong blocky (6.98%). The highest coefficient of variation was observed in stem thickness (83.10%) which was followed by the number of fruits per plant (43.16%) and no. of the primary lateral shoot (34.48%). Considering the fruit yield per plant, early flowering, fruit length the germplasm AC-193, IAH-284, IA-43, AMA-395, AC-147, AHI-37 and TRMR-14 were found better which can be used in the future improvement or breeding programs.
CHARACTERIZATION OF INDIAN SPINACH GERMPLASM DURING 2020-21
M F KHATUN, R A CHHANDA, M S UDDIN, S RAHMAN, M G HOSSAIN, Q M AHMED AND M S NAHAR
The study was conducted at the experimental field of Plant Genetic Resources Centre (PGRC) of Bangladesh Agricultural Research Institute (BARI), Gazipur, during the Kharif season 2020-21. Forty-two germplasm of Indian spinach were characterized in this study. Most of the qualitative characters showed a few variations. The variations were found in leaf colour, leaf shape, stem colour, stem shape, leaf margin colour, leaf margin, flower colour as well as seed colour and seed size. Among the germplasm maximum variation was observed in seed colour whereas maximum color was observed in dark brown (42.86%), Brown (33.34%), light brown (9.52%), reddish-brown (9.52%), and straw (9.52%). Plant growth habit was observed in three types such as twining (26.19%), procumbent (59.52%), and bushy (14.29%). The stem colour showed green (76.19%), dark red (15.10%), and light red (9.52%). Among the germplasm, the stem shape mostly provided angular type (95.24%). The green-colored leaf was the highest (32 germplasm) followed by light green (4 germplasm) and dark green (6 germplasm). The highest coefficient of variation 43.69% was found in leaf weight (leaf yield) which was followed by stalk weight 41.67% which indicates stalk yield per meter vine. Three distinct clusters observed by cluster analysis where cluster 1 was found to better due to the highest mean value.
CHARACTERIZATION OF CHILLI GERMPLASM
M F KHATUN, N JAHAN, N JAHAN, N PERVIN AND M S NAHER
The study was conducted in the experimental field at the Plant Genetic Resources Centre (PGRC) of BARI Gazipur, during the winter season of 2021-22. 35 qualitative characters and 12 quantitative characters were recorded from 38 genotypes to characterize the chilli germplasm as per documented followed by IBPGR descriptor. Qualitative variation was observed in overall leaf color, flower color, fruit color at the immature stage and fruit shape at the blossom end. The maximum variation was observed in leaf color such as light green (21.05%), green (47.37%), dark green (28.95%), and light purple (2.63%). Variation was also found in corolla colour where white (65.78%), light yellow (28.95%), purple (26.32%), and white with a purple margin (2.63%). The fruit shape at the blossom end was found in four categories such as pointed (63.16%), blunt (26.32%), sunken (7.89%), and sunken and pointed (2.63%). The highest coefficient of variation was recorded in stem length (56.7%) which was followed by fruit width (49.5%), and individual fruit weight (49.42%). Considering the yield, AHM-176, AMA-199, RC-79, IAH-96, RC-81, and ATR-03 were shown superior.
N JAHAN AND M F KHATUN
The experiment was conducted with forty-one (41) germplasm of brinjal (Solanum melongena L.) in the experimental field at Plant Genetic Resources Centre (PGRC) of BARI, during winter 2021-22 to find out the variability in the germplasm. All the qualitative characters showed distinct variation among the germplasm except fruit cross section and fruit position. The maximum variation was observed in overall fruit length breath ratio and fruit calyx prickles. The highest quantitative variation was observed in fruit length (CV-43.79%), followed by individual fruit weight (CV-32.91%) and yield per plant (CV-30.12%). More of the germplasm were scattered into cluster I. Cluster II and Cluster III included ten germplasm, respectively. The maximum cluster mean value were observed in Cluster II for the characters days to 50% flowering (67.90%), plant height (97.97 cm), Individual fruit weight (107.35 g) and yield per plant (2.51%). The first PC explained 27.28% of the total variability and the second PC explained 22.36% of the variation among 41 brinjal germplasm. In biplot, the brinjal germplasm NF-20, NF-13, NF0-7, NF-4, NF-25, NF-16 and NF-2 etc. were separately isolated from the others and they were away from centroid. These results showed their uniqueness and divergence of the germplasm in respect to the measured traits.
CHARACTERIZATION OF MUSKMELON GERMPLASM
N JAHAN, Q M AHAMED, M F KHATUN, N PERVIN AND M M ALI
Forty-two muskmelon (Cucumis melo) germplasm were studied at the Plant Genetic Resources Centre of BARI, Gazipur during February-June 2022 to identify the variations among the germplasm. Variation was found on fruit shape, predominant fruit skin colour, secondary fruit skin colour, primary colour of immature fruit, secondary colour of immature fruit, secondary skin colour pattern. In fruit character, there were globular (14.29%), flattened (19.05%); oblate (33.33%), elliptical (2.38%), pyriform (2.38%), elongated (23.81%) fruit shape were identified. In fruit cracking habit, 28.57% germplasm showed deep cracking, 9.52% intermediate and 33.33% superficial. The maximum 52.38% germplasm showed pale orange fruit flesh colour. The maximum 69.05% germplasm contained grainy-firm fruit flesh texture. All germplasm had contained internal aroma. The range of fruit length was 9.50 cm to 51 cm and breath was 5 cm to 18 cm. The average value of flesh thickness was 2.38 cm. The minimum fruit weight was 0.33 kg and the maximum was 5.46 kg. Maximum coefficient of variation 73.53 % was estimated in fruit weight followed by fruit length (46.35%).
CHARACTERIZATION OF SPINACH GERMPLASM
N JAHAN, N JAHAN AND M R MOLLA
Fifty-one germplasm of spinach (Spinacia oleracea L.) were evaluated for several qualitative and quantitative traits at the Plant Genetic Resources Centre of BARI, Gazipur during rabi 2021-22 to know the variability in the germplasm. Variation was found in all the characters except Inflorescence color and seed type. Leaf shape was exhibited as elliptic, broad elliptic, ovate and broad ovate where elliptic shape was found in maximum germplasm (64.71%) where as 11.76% germplasm had broad elliptic leaf shape and 13.73% had ovate leaf shape. Leaf texture of the germplasm exhibited as smooth (90.20%) and slight crinkled (9.80%). The highest quantitative variation was observed in edible leaf weight per plant (CV-30.59%) followed by petiole length (CV-22.21%) and number of lateral branches (CV-19.40%). Germplasm TRMR-95, NT-34, TRMR-136, NRI-121 had longer leaf and NRI-121, TRMR-136, RC-139, TRMR-12 and NT-33 had broader leaf. Germplasm RC-139 had highest number of lateral branches (9). The germplasm NQ-68 required 85 days for bolting which was followed by TRMR-136 (83 days) and RNF-126 (83 days) that was most important characters for spinach. The dendrogram shown that the maximum thirty-six germplasm were grouped into cluster I. Cluster IV was occupied by only one germplasm. The maximum cluster mean value were observed in Cluster II for the characters leaf length (cm), leaf width (cm) and edible leaf weight per plant (g). The first PC explained 37.10% of the total variability and the second PC explained 22.90% of the variation among fifty-one spinach germplasm. Populations with high scores for the first eigenvectors are leaf length (0.4860), leaf width (0.4827) and petiole length (0.4792) these traits were the most important contributors towards diversity of the germplasm in PC1. Considering leaf characters and bolting time, germplasm TRMR-136, TRMR-144, R-342, RNF-26, NQ-68 and RC-139 were selected for future breeding program.
CHARACTERIZATION OF AMARANTH GERMPLASM
N JAHAN, M G HOSSAIN, M R MOLLA, Q.M AHMED AND M S NAHAR
Seventy-two accessions of amaranth (Amaranthus spp. L.) were studied in augmented block design at the Plant Genetic Resources Centre (PGRC), BARI, Gazipur during winter 2021-22. All the accessions showed variations both for qualitative and quantitative characters. Qualitative variation was found in different parameters as early plant vigor, plant growth habit, compactness, shape and spininess of inflorescence, texture of stem. Moreover, different color variations were displayed in leaf (yellowish green 05.56%, green 23.61%, pinkish green 27.78%, reddish green 31.94%, red 05.56% and dark red 05.56%), inflorescence (yellowish orange 13.89%, purple 11.11%, red 02.77%, reddish green 30.56% and green 41.67%), stem (05.56% pink, 15.28% red, 54.17% reddish green and 25% green), and seed (red 15.28% and black 84.72%). In case of quantitative character, plant height ranged from 14.30-198.6cm. The accessions flourished with 50% flower within 41-109 days giving 17.40 cm long inflorescence on an average. Average seed yield per plant was 14.67 g. Nevertheless, highest CV was found in case of number of days to germination (45.58%) and lowest in leaf length (19.91%). The accessions namely, N-94, TT-99, TT-140, TT-142, TT-198, NRI-268, MRI-41, SSR-3, SSR-25, SSR-45, RC-35 and RC-151 have been recommended for using in future breeding program.
CHARACTERIZATION OF LENTIL GERMPLASM (SET-I)
K U AHAMED AND M M UDDIN
The study was conducted at Regional Plant Genetic Resources Center, Regional Agricultural Research Station, Ishurdi, Pabna during Rabi season of 2021-2022 to study the genetic diversity in lentil germplasm and to identify accession having useful traits. The experiment involved 324 lentil accessions. Variations were observed in respect of time to flowering, time to maturity, plant height, number of seeds per pod, 100-seed weight and yield per plant among lentil accessions. Days to 50% flowering was earlier in BD-3819, BD-3844, BD-4044, BD-4045, BD-4105, BD-4106, BD-5958, BD-5959, BD-5963, BD-5964, BD-6023, BD-6024, BD-6025 and BD-6027 (46 days) than the other accessions. The earlier maturity observed in BD-5998 (101 days). Variations were observed among lentil accessions in different qualitative characteristics like plant pigmentation were observed in stems, leaves and flowers. Seedling stem pigmentation was observed present for 34.26% and absent 65.74%. Variations in tendril length found rudimentary for 28.09% and rest accessions found in prominent maximum for 71.91%. Flower ground colour observed maximum white colour for 75.93%, white with blue veins for 16.05%, blue for 6.17%, violet for 7.84% and rest accessions showed pink for 0.62%. Pattern of testa showed absent for 0.31%, dotted for 89.20%, spotted for 2.47%, marbled pattern of testa showed for 5.56% where rest accessions 2.47% showed complex. Colour pattern on testa observed absent for 0.31%, black for 0.93%, grey for 11.11% where brown colour pattern of testa showed for 87.65%. Cotyledon colour found yellow for 0.31% and rest 99.69?cessions showed orange-red colour. Pest and disease susceptibility found low for 23.77%, medium showed for 43.52% and rest 32.72?cessions showed high susceptibility to pest and disease which affected by aphid and stemphylium blight disease symptom in stem and leaves. Variations were observed in different quantitative characteristics as numbers of pods per plant varied from 41.50 to 388.00, maximum number of pods per plant recorded 201.00 to 388.00, moderate for 100.00 to 199.00 and rest accessions found lowest 41.50 to 99.00 number pods per plant and BD-10726 produced significantly the highest number of pods per plant (388.00). Seed yield varied from 1.13 to 14.88g per plant, where BD-3829, BD-3904, BD-4033, BD-4056, BD-4098, BD-5960, BD-5980, BD-6000, BD-6008 and BD-10726 showed high yielding 10.02 to 14.88 g per plant, moderate yielding 6.03 to 9.83 g per plant and rest found lowest yielding 1.13 to 5.98 g per plant. The highest seed yield 14.88 g per plant was recorded from BD-3904 lentil accession and the lowest yield 1.13 g per plant found from BD-4078 lentil accession.
CHARACTERIZATION OF LENTIL GERMPLASM (SET-II)
M G HOSSAIN, M R MOLLA, I AHMED, N JAHAN, M F KHATUN, Q M AHMED, S RAHMAN, R A CHHANDA AND M M. ALI
Characterization of collected germplasm helps to estimate diversity among the germplasm. germplasm. Fifty-three accessions of lentils (Lens culinaris) were characterized at PGRC research field, BARI, Gazipur during rabi 2021-22 season following augmented RCB experimental design to identify the important traits. Diversity both for qualitative and quantitative characters were observed in all characters except number of seeds per pod and number of flowers per peduncle. Variations in leaf pubescence and seedling stem pigmentation were observed. Different leaflet size viz. small for 15 accession (28.30%), medium for 37 accessions (69.81%), and large for 1 accession (1.89%) were recorded. Dissimilarity in ground color of testa viz. grey, brown and black were identified. Significant variations were observed in days to 50% flowering, days to 90% maturity, plant height, number of primary branches, number of pods per plant, 100-seed weight and seed yield per plant among the tested accessions. Early flowering was observed in accession NSQR-07 and NSQR-23 (47 days) while the earlier maturity was observed in NSQR-23 that took only 98 days. The accessions NQR- 17 possessed the maximum number of pods per plant (263). NSQR-08 produced the highest number of primary branches per plant (4.4). The tallest plant was found in NSQR-13 (39.2cm) while the dwarf was in NRI- 95 (20.6cm). The highest seed yield (40.19 g/plant) was recorded from NSQR-06 and the lowest (3.63 g/plant) from NRI-101 and N-101 (3.65 g/plant) lentil accession.
NUTRITIONAL AND MORPHOLOGICAL CHARACTERIZATION OF TOMATO GERMPLASM FOR VARIETAL IMPROVEMENT
S RAHMAN, Q M AHMED, M S NAHAR, M M ALI
The experiment was conducted with 76 germplasm including three check variety of tomato (Solanum lycopersicum) in the experimental field at Plant Genetic Resources Centre (PGRC) of BARI, Joydebpur, Gazipur, in 2019-20 to 2020-21 to find out the variability in the germplasm. Qualitative characters showed distinct variation among the germplasm except hypocotyl pubescence (present), anthocyanin colour of leaf veins (normal), skin colour of ripe fruit (yellow), seed shape (ovate). The maximum variation was found in ‘predominant fruit shape’ followed by ‘fruit size’, fruit shoulder shape’ and ‘flesh colour of pericarp (interior)’. Six categories of predominant fruit shape were observed such as flattened (53.95%), slightly flattened (5.26%), rounded (14.47%), highly rounded (17.11%), hard shape (5.26%) and cylindrical (3.95%). Fruit shoulder shape was observed in four categories viz. flat (17.11%), slightly depressed (39.47%), moderately depressed (13.16%) and strongly depressed (30.26%). Interior flesh colour of pericarp was exhibited as green (42.11%), yellow (2.63%), pink (44.74%) and red (10.53%). Quantitatively highest variation was observed in number of locules (CV- 45.82%) which was followed by fruit weight (CV-39.94%) and yield per plant (CV-34.99%). Organoleptic test showed sweetened, sower-sweetened, crispy and Juicy tomatoes. Currently nutritional analysis is ongoing. Among the germplasm the sweetest tomatoes were SS-21, BD-7257, BD-7290.The germplasm BD-7756, RISA-14413 and SS-18 might be considered as the best yielder among the studied germplasm.
CHARACTERIZATION OF BLACKGRAM GERMPLASM
M I RIAD and M M KADIR
Blackgram (Vigna mungo L.), one of the most important pulse crops belongs to the family Leguminosae. Eighty-eight (88) germplasm of blackgram were characterized at plant Genetic Resources Centre (PGRC), RARS, Jamalpur during 2021-2022. In qualitative variation was predominantly present in most of the characters except petiole colour, calyx colour, pod curvature, seed colour and seed shape. The germplasm were analyzed for 35 qualitative and quantitative characters. In quantitative traits, early flowering and maturity was observed in germplasm BD-6837 (42 days and 59 days). The characters number of primary branches/plant, number of pods/plant, pod length, number of seeds/pod, 100 seed weight and seed yield/plant batter performance was found in germplasm BD-6805 (10), BD-6797 (50), BD-6806 (4.82 cm), BD-6834 (7), BD-6866 (5.72 g) and BD-6797 (13.46 g). The character plant height showed highest standard deviation (30.07) and coefficient of variation (CV %) (40.51%) and mean (172.47) was found in the character days to maturity.
CHARACTERIZATION OF GRASSPEA GERMPLASM (SET-I)
M S KOBIR, N JAHAN, M R MOLLA, S RAHMAN, M S. NAHAR, K U AHAMED AND M M ALI
The study was conducted at Plant Genetic Resources Center, Regional Agricultural Research Station, BARI, Jashore during Rabi season of 2021-22 to identify the important traits of grasspea accessions. The experiment involved 200 grasspea accessions. Variations among grasspea accessions were observed in qualitative characteristics like seedling vigor, plant growth rate stage-I, plant growth rate stage-II, plant growth habit, stem colour, leaf colour, leaflet shape, flower size, flower colour, pod shape, seed size and seed colour. Plant growth rate stage-I was observed low for 60 accessions (30%), medium for 77 accessions (38.5%) and high for 63 accessions (31.50%). Plant growth habit was recorded erect for 17 accessions (8.5%), semi-erect for 14 accessions (7%), spreading for 88 accessions (44%) where prostrate showed 78 accessions (39%). Flower colour was observed blue for 198 accessions (99%), pink for 2 accession (1%). Seed size showed small for 11 accessions (5.5%) and medium for 189 accessions (94.5%). Variations were observed in respect of days to first flowering, days to 50% flowering, days to maturity, plant height, number of seeds per pod, 100-seed weight, seed yield plot-1 and seed yield ha-1 among grasspea accessions. BD-4350 and BD-5340 produced highest number of pods per plant (124). The lowest days (58) to first flowering was observed in BD-3378, BD-4756, BD-4860, BD-4978. The lowest days to 50% flowering was observed in BD-4759, BD-4756 and in BD-4978. The highest seed yield was observed in BD-3629 (3844.43 kg ha-1) which was followed by BD-4753 (3700 kg ha-1), BD-4768 (3615.55 kg ha-1), BD-4839 (3582 kg ha-1), BD-3718 (3573.33 kg ha-1), BD-3795 (3526.67 kg ha-1), BD-4743 (3504.45 kg ha-1) and BD-4754 (3437.77 kg ha-1) whereas the lowest seed yield was found in BD-5243 (1142.3 kg ha-1) and these better performing genotypes may be considered as better accessions. These accessions may be used in grasspea improvement programme.
M HOSSAIN AND A K SHAHA
To assess the genetic diversity of 200 Grasspea germplasm a field experiment was conducted in the experimental field of RARS, Burirhat, Rangpur during the winter season of 2021-22. A high degree of diversity was found among the pea germplasms both for qualitative and quantitative traits. All the studied qualitative characters showed distinct variation among the germplasm except leaf pubescence and flower colour. The maximum variation was observed in stem colour. The maximum percentage of seedling (47 %) was found vigorous, then 41.5% seedling was found intermediate and 11.5% was found poor, respectively. In respect of stem colour, green colour of stem was found the maximum of 55.55 %, then 29.5% was light green and 15 % gemplasm was containing dark green stem colour, respectively. The highest quantitative variation was observed in the number of pods per plant (CV-50.29 %). The germplasm BD-5669 produced the highest number of seed (6.60) per pod. The highest seed yield (28.70 g) was recorded from the germplasm BD-5648. Therefore, selection of these genotypes might play a significant role for future breeding program.
CHARACTERIZATION OF GRASSPEA GERMPLASM (SET-III)
K U AHAMED AND M M UDDIN
The study was conducted at Regional Plant Genetic Resources Center, Regional Agricultural Research Station, Ishwardi, Pabna during Rabi season of 2021-2022 to identify the important traits of grasspea accessions. The experiment involved 202 grasspea accessions. Variations were observed in respect of days to first flowering, days to 50% flowering, days to maturity, plant height, number of seeds per pod, 100-seed weight and yield per plant among grasspea accessions. The first flowering initiation was earlier in BD-5741(49 days) than the other accessions and days to 50% flowering was determined by recording earlier in four accessions such as BD-5715, BD-5745, BD-5746 and BD-5753 (62 days). The earlier maturity was found in BD-5798 (105 days) than the other accessions. Variations were observed among grasspea accessions in different qualitative characteristics like pigmentation found in stems, leaves and flowers. Stem colour was showed light green for 18.32%, green for 75.25%, purple-green for 4.95% and rest was purple for 1.49%. Four types of plant growth habit were found as recorded erect type for 0.99%, semi-erect for 20.30%, spreading for 45.05% where prostrate showed 33.66%. Three types of number of leaflets per leaf were observed one pair for 13.37%, two pair for 76.73% and rest accessions showed more than two pair for 9.90%. Different types of leaflet shape were found as linear for 9.41%, lanceolate for 39.60% and rest 61 accessions showed ovate-lanceolate for 30.20%. Two types of flower colour were observed blue for 97.52% and rest accessions showed violet flower colour for 2.48%. Seed shape was observed oblate or flattened for 0.49%, square for 13.37%, triangular for 5.94%, obtriangular for 3.46% and spherical type seed shape showed 0.49%. Seed size was observed small for 3.96%, medium for 42.08% and rest accessions showed large for 53.96%. Seed coat pattern was recorded absent for 0.99%, marbled for 56.93%, dotted for 41.58% and rest accessions showed mixture for 0.50%. Different cotyledon colour was observed among accessions as yellow for 38.61% and rest showed orange for 61.39%. Different biotic stress susceptibility on pest and diseases were found among 202 grasspea accessions. Variations were observed in different quantitative characteristics among grasspea accessions as number of secondary branches per plant varied from 4.33 to 15.33, 118 accessions showed maximum number of secondary branches per plant (10.33-15.33). Nnumber of pods per plant varied from 36.50 to 115.00, while 15 accessions showed highest number of pods per plant (100.00−115.00), 84 accessions gave moderate number of pods per plant (70.50−99.00) and rest 103 accessions produced lowest number of pods per plant (36.50−69.00). Yield varied from 2.14 g to 28.11 g per plant, where BD-5737, BD-5719, BD-5752, BD-5762, BD-5819, BD-5837, BD-5888, BD-5923, BD-5933 and BARI Grasspea-2 showed high yielding (20.09−28.11 g/plant), 139 accessions showed moderate yielding (10.14−19.92 g per plant) and the rest 53 accessions showed low yielding (2.14−9.79 g per plant). The highest seed yield (28.11 g per plant) was found from BD-5737 grasspea accession and the lowest yield (2.14 g per plant) found from BD-5889 grasspea accession.
CHARACTERIZATION OF MUSTARD GERMPLASM
M A A MALEK, M R MOLLA, S RAHMAN, M RABI AND M R AHMAD
This experiment was conducted at the hill valley of Hill Agricultural Research Station, Khagrachari. Quantitative characters showed variation in days to 50% flowering ranged from 38 to 56 days, pod formation ranged from 45 to 61 days, harvesting ranged from 81-97 days. Accession BD-7105 showed first flowering and BD-7809 & BD-9292 were late flowering. Accession BD-7125 showed maximum plant height 170.4 cm. The seed of all accessions will store in cold storage of PGRC at Gazipur for further evaluation.
CHARACTERIZATION OF RED AMARANTH (Amaranthus spp.)
S RAHMAN, N JAHAN, N JAHAN, M S NAHAR AND M ALI
The present investigation was conducted in the experimental field of Plant Genetic Resources Centre (PGRC), BARI to evaluate and characterize seventy-six diverse germplasm of red amaranth collected from different districts in Bangladesh. The characterization results revealed wide range of variation for most of the qualitative morphological parameters except plant growth habit (erect), stem colour (red), stem surface (ridged) and inflorescence shape (semi-drooping). The maximum variation observed in early plant vigour, leaf colour, inflorescence colour. Quantitatively, the highest variation was observed in petiole length (CV- 35.14%).
CHARACTERIZATION OF OKRA GERMPLASM
N JAHAN, Q M AHMED, N PERVIN, R AFROZ AND M S NAHAR
An experiment was conducted with 37 germplasm of okra (Abelmoschus esculentus L.) in the experimental field at Plant Genetic Resources Centre (PGRC) of BARI, Joydebpur, Gazipur, during summer 2021-22 to find out the variability in the germplasm. Qualitative characters showed distinct variation among the germplasm except shape of epicalyx segments, position of fruit and seed shape. The maximum variation was found in leaf shape followed by leaf color, fruit shape, mature fruit color and early plant vigour. Quantitatively highest variation was observed in yield per plant (CV- 34.46%) followed by number of epicalyx segments (CV-22.36%) and plant height (CV-22.22%). Considering maximum number of fruits per plant the promising germplasm were identified as. NQ-10, NRI-289, NSR-98, NSR-133, NSR-158, SRS-63, SSR-11, AMA-40, AMA-126, MAH-59, NQR-20, NQR-40, RAI-157 and SNQR-44 for future breeding programs.
R AFROZ AND M M ALI
One hundred fifty-eight (158) germplasm of mugbean (Vigna radiata) were characterized at plant Genetic Resources Centre, BARI, Gazipur during 2018-19. Qualitative variations were observed; deltoid 66.46% and ovate 33.54% were exhibited in the terminal leaflet shape followed by sparse 40.51%, medium 52.53%, abundant 6.96% such variation found from leafiness. Petiole length such as short 58.22% and medium 41.77%; raceme position such as mostly above canopy 45.57% and intermediate 54.43% were observed. The maximum range of number of pod per plant was 70.72 to 1201.15 and mean 406.21. In plant height, mean 31.54 cm and range 18.53 to 47.35cm, in number of seed per pod range 5.8 to 13.74 and mean 10.42. The maximum coefficient of variation 78.22% was obtained from seed yield per plant followed by No. of pod per plant 65.4%, no. of primary branches per plant 24.47%. The frequency distribution observed higher on no of primary branch/plant, Plant height and no. of seed /pod. Unique selection and trait information is available within the population studied which may offer crop improvement opportunity. In the same time 158 germplasm evaluate under waterlog condition at vegetative stage until four days and 94 germplasm survived. During 2021-2022 the experiment was carried out with 90 germplasm of mung bean with 4 check varieties, all germplasm were evaluated under waterlog system at flowering stage. In vegetative stage 94 germplasm survive under waterlog condition until 4 days and in flowering stage only 15 germplasm showed tolerant character which were sub merged in water reservoir for 72 hours. Further evaluation will be needed in field condition.
SCREENING OF SESAME GERMPLASM AT SEEDLING STAGE FOR WATERLOGGING STRESS TOLERANCE
M R MOLLA, I AHMED, NASRIN JAHAN, M M ROHMAN, S H HABIB, M S NAHAR AND M M ALI
Waterlogging is an illustrious abiotic stress and the constrictions it enforces on plant roots have negative effects on growth and development. This study was undertaken to investigate waterlogging stress tolerant potential in sesame (Sesamum indicum L.) at seedling stage. Thirty days old seedlings of 42 sesame genotypes were exposed to waterlogging stress maintaining water height 3–5 cm over the soil surface artificially for 3-4 days. This duration (31–34 DAE) was termed as waterlogging period, and subsequent withdrawal of waterlogging condition (35-41 DAE) was regarded as a recovery phase. Based on their survival performance, among the 42 genotypes of sesame, acute decreasing of survival and increasing of death percentage was observed in most of the genotypes under waterlogging and recovery. The genotype BD-10659 showed the strong tolerance whereas BD-10660, BD-7026 and BD-11637 expressed moderate tolerance under waterlogging stress.
MOLECULAR CHARACTERIZATION OF LANDRACES OF CHILLI IN BANGLADESH
M R MOLLA, I AHMED, S RAHMAN, R ARA, M S NAHAR AND M M ALI
Microsatellites combine a number of characteristics of a perfect molecular marker, and they are increasingly employed in a variety of plant genetic investigations and applications. Characterization of chilli genotypes on the basis of DNA fingerprinting has become an efficient tool to link genotypic variation. This work is reporting the utilization of a set of 10 previously developed microsatellite (SSR) markers for the identification and discrimination of 22 landraces. The modified SDS and phenol: chloroform: IAA protocol found to be cost effective and latex free high quality genomic DNA was obtained successfully. All microsatellite markers were found to be polymorphic. Variation was found in number of alleles, allele frequency, observed and expected heterozygosity. Using 10 primers across 22 genotypes a total of 41 alleles with an average number of 4 alleles per locus were found of which CAMS-647 showed the highest number of alleles (6) (size ranging from 191 to 275 bp) followed by 5 alleles (165 to 194 bp and 239 to 289 bp) were detected at the loci CAMS-679 and CAMS-117 respectively. The primer CAMS-647 also yielded the highest number of PIC value (0.776). Genetic differentiation (Fst) values were found in the ranges from 0.243 to 1.000 with an average of 0.728 and gene flow (Nm) values ranged from 0.000 to 0.781 with an average of 0.094. Broad genetic base was found among the chilli genotypes. Over all Nei’s genetic distance value from 0.124 to 0.999 among 231 pair resulting as a means of permutation combination of 22 chilli genotypes. In the UPGMA dendrogram, among 22 genotypes of chilli, 20 grouped in cluster “A” and other two in cluster “B”. Bindu morich, Amoina morich, Noadaria morich, Bine morich, Balujhuri morich and Halda morich were identified as distinct landraces of Bangladesh.
MOLECULAR CHARACTERIZATION OF LANDRACES OF GUAVA IN BANGLADESH
M R MOLLA, H BARUA, I AHMED, Q M AHMED, M S NAHAR AND M M ALI
Five released varieties of guava viz., BARI Peyara-1, BARI Peyara-2, BARI Peyara-3, BARI Peyara-4, IPSA Peyara-1; three exotic germplasm viz.,Thai Peyara-3, Thai Peyara-5, Thai Special; 10 landraces PG Sit-001, PG Sit-002, PG Sit-003, PG Sit-004, PG Muk-004, PG Muk-005, Lata, Poltola, Purnamandali, Poly-peyara and 15 advance line viz., PG Hat-004, PG Hat-009, PG Hat-010, PG Hat-011, PG Hat-012, PG Hat-013, PG Hat-014, PG Hat-015, PG Hat-016, PG Hat-017, PG Hat-018, PG Hat-019, PG Hat-020, Swarupkathi-1, Swarupkathi-3 were characterized with a view to identifying degree of molecular variation of guava within genotypes, and to establish a permanent database for documentation of guava. Molecular characterization was carried out with SSR markers. Using 20 primers across 33 genotypes a total of 62 alleles with an average number of 3.1 alleles per locus were found of which mPgCIR139 showed the highest number of alleles (5) (size ranging from 201 to 296 bp). However, the lowest number of allele (2) was observed in the locus mPgCIR25, mPgCIR27, mPgCIR48, mPgCIR180, mPgCIR191 and mPgCIR111, respectively. The polymorphic information content (PIC values) ranged from 0.316 to 0.709, with a mean value of 0.573 for all loci. Of 20 SSR primers, it was identified 11 (55.90%) highly informative (PIC value ≥0.5). The higher level of heterozygosity indicated that greater diversity of genotypes used in the present study. Band patterns corresponding to individual genotype have been identified to discriminate the genotype. The genotypes presented genetic distances between 0.048 and 0.997. The dendrogram generated from UPGMA cluster analysis broadly placed 33 guava genotypes into two major clusters, “A” and “B” in which 21 genotypes grouped in cluster “A” and other 12 grouped in Cluster “A”.
CONSERVATION OF GERMPLASM IN ACTIVE AND BASE COLLECTION
S RAHMAN, Q M AHMED, M S NAHAR AND M ALI
Plant Genetic Resources Centre (PGRC) acts as a germplasm store house of the BARI mandated crops viz. cereals, pulses, oilseeds, vegetables, fruits, spices and other crops etc., since 1987. The accessions were conserved in medium-term storage (at 4 to 60C) and long-term storage (at -18 to -220C). Viability (germination %), quantity and moisture were checked before conservation. Accessions having less than 80% viability and/or less quantity of seeds were regenerated. Generally, the seeds were dried at 6-8% moisture content before storing. Till now, the Centre has conserved 12494 (twelve thousand four hundred ninety-four) accessions of 82 different crops in its gene bank. Among them, 1775 accessions were cereals, 3553 pulses, 616 oilseeds, 487 spices, 4780 vegetables, 283 fruits and 57 other crops. In 2021-22, a total of 146 germplasm was assigned as new accession and were conserved in gene bank of PGRC.
MONITORING OF GERMPLASM IN ACTIVE AND BASE COLLECTION
S RAHMAN, Q M AHMED, M S NAHAR AND M ALI
The monitoring of 1076 accessions from different year (batch references) among 14 important crops viz. 167 cucumber (167), yard-long bean (394), radish (117), horse gram (67), pigeon pea (83), jute (25), sunhemp (7), tobacco (29), barley (100), buck wheat (10), rosel (16), napa sak (42), Kangkong (17) and zirani (2) were tested in 2021-22 by germination test. Among the monitored germplasm 570 accession from active collection and 506 accessions were from base collection. The viability test was conducted on germination paper, sands and pulverized gravels. Combining all data, it was found that 81-100% germination was higher and it was followed by 41-80% germination and less than 40% germination in active collection. Similar trend was found in base collection. Altogether, base collection performance was better over the active collection. The accessions having less than 80% germination and or less quantity will be regenerated in the following year.
DISTRIBUTION OF GERMPLASM
S RAHMAN, Q M AHMED, M R MOLLA, M S NAHAR AND M ALI
Germplasm distribution is one of the important activities of Plant Genetic Resources Centre (PGRC). The centre distributed 1294 accessions of 23 crops among the researchers, MS and PhD students, plant breeder, horticulturist and teachers of different Universities and Institutes for conducting research on varietal improvement as well as screening and evaluation like diseases, insect screening, salinity stress, mutation breeding, abiotic stress, fibre production, draught tolerant and molecular diversity analysis during 2021-22. Among the germplasm, 335 accessions were cereals (Buck wheat, maize, sorghum, and wheat), 261 oil seeds (Linseed, sesame, soybean and sunflower), 103 pulses (mung bean, cowpea and pigeon pea), 40 spices (chilli and black cumin), 555 vegetables (Bitter gourd, bottle gourd, brinjal, cucumber, okra, hyacinth bean, pumpkin and tomato). Ten to hundred seeds or 5-10 g seeds per accession were supplied to the users.
MULTIPLICATION OF NEWLY COLLECTED GERMPLASM OF HYACINTH BEAN, BRINJAL, OKRA AND BITTER GOURD
N PERVIN
The experiment was conducted at the experimental field of Plant Genetic Resources Centre, BARI, Gazipur, during rabi and kharif 2021-22 to multiply the newly collected germplasm of hyacinth bean (9), brinjal (12), okra (12) and bitter gourd (4). All the germplasms were planted from 26 October, 2021 to 3 March, 2022 with recommended practices to get adequate quantity of seed for future use. Each germplasm were harvested in time. Proper procedure was followed during drying, cleaning and curing the seed. Some qualitative data were recorded to assess the overall performance. Seeds obtained from newly collected germplasm were conserved in the gene bank.
REGENERATION OF CONSERVED ACCESSION OF FRENCH BEAN AND BUCK WHEAT
N PERVIN
The experiment was conducted at the experimental field of Plant Genetic Resources Centre, BARI, Gazipur, during rabi 2021-22 to regenerate the conserved accession of French bean (10) and Buck wheat (3). All the germplasms were planted from 25 November, 2021 to 27 November, 2021 with recommended practices to get adequate quantity of seed for future use. The crops were harvested at time to time for each germplasm. Proper activities were done during drying, cleaning and curing the seed. Some qualitative data were recorded to assess the overall performance. Seeds were obtained from newly collected germplasm and conserved accession and conserved in the gene bank.
REGENARATION OF MUSKMELON GERMPLASM
N JAHAN AND M R MOLLA
The regeneration of twenty-three muskmelon (Cucumis melo) germplasm was conducted in the experimental field at Plant Genetic Resources Centre (PGRC) of BARI, Gazipur, during February–June, 2022. Germplasm regeneration leads to get the sufficient seed quantity with increase viability for future use. The most important qualitative and quantitative characters were recorded to know the variations among the germplasm. After completing all the post-harvest operations, the seeds were conserved properly for future study.
REGENARATION OF SOYBEAN GERMPLASM
N JAHAN AND M R MOLLA
The regeneration of fourteen soybean (Glycine max L.) germplasm was conducted in the experimental field at Plant Genetic Resources Centre (PGRC) of BARI, Gazipur, during January – May, 2022. Germplasm regeneration leads to get the sufficient seed quantity with increase viability for future use. The most important qualitative and quantitative characters were recorded to know the variations among the germplasm. After completing all the post harvest operations, the seeds were conserved properly for future study.
REGENERATION OF INDIAN SPINACH GERMPLASM
M F KHATUN
The regeneration experiment was conducted at the research field of Plant Genetic Resources Centre (PGRC) of Bangladesh Agricultural Research Institute (BARI), Gazipur during the Kharif season of 2020-21. Thirteen germplasm were taken for regeneration. Variations were observed in both qualitative and quantitative traits among the germplasm. Some of the qualitative and quantitative data were recorded and an adequate amount of seeds were harvested for conservation and future use.
REGENERATION OF RIDGE GOURD GERMPLASM
M F KHATUN, Q M AHMED AND N JAHAN
The experiment was conducted at Plant Genetic Resource Centre, BARI, Gazipur during the Kharif season 2021-22. Sixty-two germplasm were regenerated to increase sufficient seed for conservation. A few qualitative and quantitative data were recorded. Among the collected data, the maximum variations were observed in fruit shape and fruit colour. Different types of fruit shapes were found which was oblong blocky, elongate slim, elongate elliptical, elongate tapered, elliptical and pyriform. In the color of the fruit were found light green, green and dark green. Some important qualitative and quantitative characters were recorded during the regeneration of the germplasm. After completing the study, the sufficient seeds were conserved for distribution and other research purpose.
CONSERVATION OF GERPLASM IN FIELD GENE BANK
M G HOSSAIN AND M M ALI
Many perennial crops and horticultural species are either difficult or impossible to conserve as seeds because they produce recalcitrant seeds or reproduce vegetatively. Hence, they should be conserved as live plants in Field Gene Banks (FGB). FGBs provide an opportunity for easy and ready access to conserved material for research and utilization. Plant Genetic Resources Centre (PGRC) of Bangladesh Agricultural Research Institute, Gzaipur maintaining a total of 281 germplasm including 213 accessions of 76 crops both indigenous and exotic germplasm in its field gene bank. In 2021-22, new 06 germplasm of 5 crops were collected from different district which has been maintaining in field gene. The fruits germplasm are mango litchi, banana, guava, jackfruit, jujube, aonla, bael, bilimbi, bullocks heart etc. The vegetables germplsm are taro, yam, elephant foot, drumstick etc. The exotic germplasm are rambhutan, pear, tamarind, coffee, passion fruit, dragon fruit and gynura etc. The intercultural practices were done as and when necessary. The field gene bank has been maintained since 1985 and continued for the following years.
DATA BASE DEVELOPMENT AND DATA ENTRY FOR GERMPLASM DOCUMENTATION
R AFROZ, Q MARUF AND M M ALI
The total four thousand one hundred (4107) passport, twelve thousand one hundred thirty-one (12131) conservation and some characterization information of germplasm were recorded under documentation system. The information of germplasm on conservation, characterization, regeneration and distribution has been in progress. This database system could be used as an inventory for Genebank accessions, identifying new crops for explorations and developing strategies for conservation and utilization. The proper documentation of plant genetic resources is required to properly conserve, manage and use biodiversity of plant species. For information of germplasm documentation is important for the research. PGRC/BARI has been created a new database information software with a powerful, flexible, easy-to-use of the information of plant genetic resource (PGR). A core set of web services, MySQL has been adopted for data entry and editing forms. The impact of system use will be evaluated by users during and following database implementation and utilization.